上海 外菜 vx

您好,欢迎来到YEASEN!
400-6111-883
| |
400-6111-883
Recombinant Human Wnt -3a protein
产品货号:92276ES60
产品规格:100 μg
产品价格:¥ 4965.00
类别:细胞因子

说明书下载

  • Product Information

     

    Product Name

    Cat#

    Size

    Price¥

    Recombinant Human Wnt -3a protein

    92276ES60

    100 μg

    4965.00

    92276ES80

    1 mg

    24525.00

     

    Product Description

     

     Wnt3a, one of Wnt family members, plays key roles in regulating pleiotropic cellular functions, including self-renewal, proliferation, differentiation, and motility. The Wnt family comprises 19 human proteins, including Wnt1, Wnt2, Wnt2b (Wnt13), Wnt3, Wnt3a, Wnt4, Wnt5a, Wnt5b, Wnt6, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a (Wnt14), Wnt9b (Wnt14b), Wnt10a, Wnt10b, Wnt11, and Wnt16. These genes encode secreted glycoproteins that are rich in cysteine. Wnts can combine with cell membrane receptors that play a critical role in autocrine regulation and/or participate in paracrine modification by binding to adjacent cell membrane receptors. The signal transduction pathway mediated by Wnt genes is called the Wnt signaling pathway. Accumulating evidence has suggested that Wnt3a promotes or suppresses tumor progression via the canonical Wnt signaling pathway depending on cancer type. In addition, the roles of Wnt3a signaling can be inhibited by multiple proteins or chemicals. Human Wnt-3a shares 96% aa identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation. Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells.

     

    Product Properties

     

    Synonyms

    Wingless-type MMTV Integration Site Family, Member 3a

    Accession

    P56704

    GeneID

    89780

    Source

    293 cells-derived Human wnt-3a, Ser19-Lys352.

    Molecular Weight

    Approximately 63.3 kDa.

    AA Sequence

    SY PIWWSLAVGP QYSSLGSQPI LCASIPGLVP KQLRFCRNYV EIMPSVAEGI KIGIQECQHQ FRGRRWNCTT VHDSLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GTAAICGCSS RHQGSPGKGW KWGGCSEDIE FGGMVSREFA DARENRPDAR SAMNRHNNEA GRQAIASHMH

    LKCKCHGLSG SCEVKTCWWS QPDFRAIGDF LKDKYDSASE MVVEKHRESR GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS SHGIDGCDLL CCGRGHNARA ERRREKCRCV FHWCCYVSCQ ECTRVYDVHT CK

    Tag

    Fc

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 75% as determined by SDS-PAGE.

    Activity

    Measured by its binding ability in a ELISA method. Immobilized Human Wnt-3a at 10 μg/ml (100 μl/well) can bind bind Wnt-3a mouse monoclonal antibody.

    Endotoxin

    1.0 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 µm filtered concentrated solution in PBS.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.


    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    1. Avoid repeated freeze-thaw cycles.

    2. For your safety and health, please wear lab coats and disposable gloves for operation.

    3. For research use only!

     

    HB220512