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400-6111-883
Recombinant Mouse sRANK Ligand/TNFSF11/RANKL,His
产品货号：90630ES03
产品规格：5 μg
产品价格：¥ 815
类别：细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price（¥）

    Recombinant Mouse sRANK Ligand/TNFSF11/RANKL,His

    90630ES03

    5 μg

    815

    90630ES20

    20 μg

    2,185

    90630ES60

    100 μg

    7,865

    90630ES76

    500 μg

    19,965

    90630ES80

    1 mg

    29,855

     

    Product Description

     

    TRANCE (receptor activator of NF-kappa B ligand, also called TNF-related activation-induced cytokine [TRANCE], osteoprotegrin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. TRANCE was originally identified as an immediate early gene up-regulated by T cell receptor stimulation. The mouse TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis.

     

    Product Properties

     

    Synonyms

    soluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF，CD254

    Accession

    O35235

    Gene ID

    21943

    Source

    E.coil-derived mouse RANKL protein, Pro143-Asp316 with His tag at the C-terminus

    Molecular Weight

    Approximately 19.4 kDa.

    AA Sequence

    MPAMMEGSWLDVAQRGKPEAQPFAHLTINAASIPSGSHKVTLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPTDYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEHFYSINVGGFFKLRAGEEISIQVSNPSLLDPDQDATYFGAFKVQDID

    Tag

    His

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 98% as determined by SDS-PAGE.

    Biological Activity

    Measure by its ability to induce osteoclast differentiation in RAW264.7 cells. The ED50 for this effect is 2 ng/mL.

    Endotoxin

    < 0.1 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in 1×PBS, pH 8.0.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    1. Avoid repeated freeze-thaw cycles.

    2. For your safety and health, please wear lab coats and disposable gloves for operation.

    3. For research use only.

     

    HB220429