- IVD PCR系列
Recombinant Mouse sRANK Ligand/TNFSF11/RANKL,His
TRANCE (receptor activator of NF-kappa B ligand, also called TNF-related activation-induced cytokine [TRANCE], osteoprotegrin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. TRANCE was originally identified as an immediate early gene up-regulated by T cell receptor stimulation. The mouse TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis.
soluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF，CD254
E.coil-derived mouse RANKL protein, Pro143-Asp316 with His tag at the C-terminus
Approximately 19.4 kDa.
Sterile Filtered White lyophilized (freeze-dried) powder.
> 98% as determined by SDS-PAGE.
Measure by its ability to induce osteoclast differentiation in RAW264.7 cells. The ED50 for this effect is 2 ng/mL.
< 0.1 EU per 1μg of the protein by the LAL method.
Lyophilized from a 0.2 μm filtered concentrated solution in 1×PBS, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.