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400-6111-883
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400-6111-883
Recombinant Mouse sRANK Ligand/TNFSF11/RANKL,His
产品货号:90630ES03
产品规格:5 μg
产品价格:¥ 815
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Mouse sRANK Ligand/TNFSF11/RANKL,His

    90630ES03

    5 μg

    815

    90630ES20

    20 μg

    2,185

    90630ES60

    100 μg

    7,865

    90630ES76

    500 μg

    19,965

    90630ES80

    1 mg

    29,855

     

    Product Description

     

    TRANCE (receptor activator of NF-kappa B ligand, also called TNF-related activation-induced cytokine [TRANCE], osteoprotegrin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. TRANCE was originally identified as an immediate early gene up-regulated by T cell receptor stimulation. The mouse TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis.

     

    Product Properties

     

    Synonyms

    soluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF,CD254

    Accession

    O35235

    Gene ID

    21943

    Source

    E.coil-derived mouse RANKL protein, Pro143-Asp316 with His tag at the C-terminus

    Molecular Weight

    Approximately 19.4 kDa.

    AA Sequence

    MPAMMEGSWLDVAQRGKPEAQPFAHLTINAASIPSGSHKVTLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPTDYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEHFYSINVGGFFKLRAGEEISIQVSNPSLLDPDQDATYFGAFKVQDID

    Tag

    His

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 98% as determined by SDS-PAGE.

    Biological Activity

    Measure by its ability to induce osteoclast differentiation in RAW264.7 cells. The ED50 for this effect is 2 ng/mL.

    Endotoxin

    0.1 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 8.0.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

     


    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20 for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    1. Avoid repeated freeze-thaw cycles.

    2. For your safety and health, please wear lab coats and disposable gloves for operation.

    3. For research use only.

     

    HB220429