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Recombinant Human Growth Regulated Protein-gamma/CXCL3 (Human GRO-γ/CXCL3)
产品货号：90906ES10
产品规格：10 μg
产品价格：¥ 1855
类别：细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price（¥）

    Recombinant Human Growth Regulated Protein-gamma/CXCL3 (Human GRO-γ/CXCL3)

    90906ES10

    10 μg

    1855

    90906ES60

    100 μg

    7865

    90906ES76

    500 μg

    20425

     

    Product Description

     

    Human GRO alpha, GRO beta (MIP-2 alpha ), and GRO gamma (MIP-2 beta ) are products of three distinct, non-allelic human genes. GRO beta and GRO gamma share 90% and 86% amino acid sequence homology, respectively, with GRO alpha. All three human GROs are members of the alpha (C-X-C) subfamily of chemokines and are thought to be the homologs of murine KC and MIP-2.

    The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively.

    Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

     

    Product Properties

     

    Synonyms

    GRO-gamma, MIP2-beta

    Accession

    P19876

    GeneID

    2921

    Source

    E.coli-derived Human GRO-γ,Ala35-Asn107.

    Molecular Weight

    Approximately 7.9 kDa

    AA Sequence

    ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 97% by SDS-PAGE and HPLC analyses.

    Biological Activity

    Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/mL.

    Endotoxin

    <  1 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    1.Avoid repeated freeze-thaw cycle.

    2.For your safety and health, please wear lab coats and disposable gloves for operation.

    3.For research use only.

     

    HB220331