上海 外菜 vx

您好,欢迎来到YEASEN!
400-6111-883
| |
400-6111-883
Recombinant Rhesus Macaque Interleukin-3 (Rhesus Macaque IL-3)
产品货号:90133ES10
产品规格:10 μg
产品价格:¥ 1855.00
类别:细胞因子

说明书下载

  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Rhesus Macaque Interleukin-3 (Rhesus Macaque IL-3)

    90133ES10

    10 μg

    1855.00

    90133ES60

    100 μg

    7865.00

    90133ES76

    500 μg

    20425.00

     

    Product Description

     

    Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells. Cytokines are actively involved in the host-defense system to coordinate the functional activity and the generation of effector cells. Some of these molecules function as inflammatory mediators and hematopoietic growth factors at the same time such as IL-3. IL-3 (or multi-CSF) is known as a pleiotropic cytokine with a broad spectrum of target cells and functions. IL-3 regulates the proliferation and differentiation of normal hematopoietic progenitor cells in the early stages of hematopoiesis. And IL-3 inhibits apoptosis and promotes the autonomous growth of blast cells.

     

    Product Properties

     

    Synonyms

    Hematopoietic Growth Factor, MCGF, Multipotential Colony-stimulating Factor, P-cell-stimulating Factor

    Accession

    5140

    GeneID

    706946

    Source

    E.coli-derived Rhesus Macaque IL-3, Ala20-Gln143.

    Molecular Weight

    Approximately 14.0 kDa.

    AA Sequence

    APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 98% by SDS-PAGE and HPLC analyses.

    Biological Activity

    Assay #1: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is less than 5.0 ng/mL, corresponding to a specific activity of > 2 × 105 IU/mg. Fully biologically active when compared to standard.

    Assay #2: The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 IU/mg.

    Endotoxin

    < 1.0 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.


    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    1. Avoid repeated freeze-thaw cycles.

    2. For your safety and health, please wear lab coats and disposable gloves for operation.

    3. For research use only.

     

    HB220402