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400-6111-883
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400-6111-883
Recombinant Human Neurotrophin 3(Human NT-3)
产品货号:92128ES20
产品规格:20 μg
产品价格:¥ 2,575
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

     Recombinant Human Neurotrophin 3(Human NT-3)

    92128ES20

    20 μg

    2,575

    92128ES60

    100 μg

    7,985

     

    Product Description

     

    Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

     

    Product Properties

     

    Synonyms

    HDNF ; NGF-2 ; NGF2 ; NT-3; NT3

    Accession

    Source

    E. coli-derived human NT-3 protein, Tyr139-Thr257

    Molecular Weight

    Approximately 13.8 kDa.

    AA Sequence

    YA EHKSHRGEYS VCDSESLWVT DKSSAIDIRG HQVTVLGEIK TGNSPVKQYF YETRCKEARP

    VKNGCRGIDD KHWNSQCKTS QTYVRALTSE NNKLVGWRWI RIDTSCVCAL SRKIGRT

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 90% by SDS-PAGE.

    Biological Activity

    Measured by its binding ability in a functional ELISA. Immobilized human NT3 at 2 μg/mL (100 μL/well) can bind human TrkB-Fch. The EC50 of human TrkB-Fch is 120-350 ng/mL.

    Endotoxin

    < 1.0 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.2.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    1. Avoid repeated freeze-thaw cycles.

    2. For your safety and health, please wear lab coats and disposable gloves for operation.

    3. For research use only.

     

     

    HB220415