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400-6111-883
Recombinant Human Insulin-like Growth Factor-Binding Protein 5 (Human IGF-BP5)
产品货号:92204ES08
产品规格:5μg
产品价格:¥ 815.00
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Human Insulin-like Growth Factor-Binding Protein 5 (Human IGF-BP5)

    92204ES08

    5μg

    815.00

    92204ES60

    100μg

    7865.00

    92204ES76

    500μg

    20425.00

     

    Product Description

     

    IGF-BPs control the distribution, function and activity of IGFs in various cell tissues and body fluids.  Currently, there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II.  IGF-BP5 is a 28.6 kDa, cysteine-rich, secreted protein produced by vascular smooth muscle cells.  It is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGF-I on smooth muscle cells, fibroblasts, and osteoblasts.  Data shows that IGFBP-5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells.  IGFBP-5-overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition, and retarded muscle development.  Recombinant Human IGF-BP5 is a 28.6 kDa protein consisting of 253 amino acid residues.

     

    Product Properties

     

    Synonyms

    Human IGF-BP5

    Accession

    P24593

    GeneID

    3488

    Source

    E.coli-derived HumanIGF-BP5 protein,Leu21-Glu272.

    Molecular Weight

    Approximately 28.6 kDa.

    AA Sequence

    LGSFVHCEPC DEKALSMCPP SPLGCELVKE PGCGCCMTCA LAEGQSCGVY TERCAQGLRC LPRQDEEKPL HALLHGRGVC LNEKSYREQV KIERDSREHE EPTTSEMAEE TYSPKIFRPK HTRISELKAE AVKKDRRKKL TQSKFVGGAE NTAHPRIISA PEMRQESEQG PCRRHMEASL QELKASPRMV PRAVYLPNCD RKGFYKRKQC KPSRGRKRGI CWCVDKYGMK LPGMEYVDGD FQCHTFDSSN VE

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 96% by SDS-PAGE and HPLC analyses.

    Biological Activity

    Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4 μg/mL, corresponding to a specific activity of > 2500 IU/mg in the presence of 15 ng/mL of rHuIGF-II.

    Endotoxin

    < 0.1 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM Sodium Citrate, pH 3.0.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.


    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    1. Avoid repeated freeze-thaw cycles.

    2. For your safety and health, please wear lab coats and disposable gloves for operation.

    3. For research use only.

     

     

    HB220318