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400-6111-883
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400-6111-883
Recombinant Human Acidic Fibroblast Growth Factor, 2-155a.a. (Human aFGF,2-155aa)
产品货号:91302ES10
产品规格:10μg
产品价格:¥ 815.00
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Human Acidic Fibroblast Growth Factor, 2-155a.a. (Human aFGF,2-155aa)

    91302ES10

    10μg

    815.00

    91302ES60

    100μg

    4315.00

    91302ES76

    500μg

    10215.00

     

    Product Description

     

    FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. Human FGF acidic shares 54% amino acid sequence identity with FGF basic and 17%33% with other human FGFs. It shares 92%, 96%, 96%, and 96% aa sequence identity with bovine, mouse, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist. During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1. It is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate . The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulate FGF acidic bioactivity . The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes.

     

    Product Properties

     

    Synonyms

    Human aFGF

    Accession

    GeneID

    2246

    Source

    E.coli-derived Human aFGF protein,Ala2-Asp155.

    Molecular Weight

    Approximately 17.3 kDa

    AA Sequence

    AEGEITTFTA LTEKFNLPPG NYKKPKLLYC SNGGHFLRIL PDGTVDGTRD RSDQHIQLQL SAESVGEVYI KSTETGQYLA MDTDGLLYGS QTPNEECLFL ERLEENHYNT YISKKHAEKN WFVGLKKNGS CKRGPRTHYG QKAILFLPLP VSSD

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 97% by SDS-PAGE and HPLC analyses.

    Biological Activity

    Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg in the presence of 10 μg/mL of heparin.


    Endotoxin

    < 0.1 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 2 mM EDTA, 0.5 mM DTT, 5 % Trehalose.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    1. Avoid repeated freeze-thaw cycles

    2. For your safety and health, please wear lab coats and disposable gloves for operation.

    3. For research use only.

     

    HB220304