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Recombinant Human Acidic Fibroblast Growth Factor (Human aFGF)
产品货号：91301ES10
产品规格：10μg
产品价格：¥ 815.00
类别：细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price（¥）

    Recombinant Human Acidic Fibroblast Growth Factor (Human aFGF)

    91301ES10

    10μg

    815.00

    91301ES60

    100μg

    4315.00

    91301ES76

    500μg

    10215.00

     

    Product Description

     

    FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors.

     

    Product Properties

    Synonyms

    FGF-1, ECGF, HBGF-1

    Accession

    P05230

    GeneID

    Source

    E.coli-derived Human aFGF protein,Phe16-Asp155,with an N-terminal Met.

    Molecular Weight

    Approximately 16.0 kDa.

    AA Sequence

    MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 95 % by SDS-PAGE and HPLC analyses.

    Biological Activity

    Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg in the presence of 10 μg/mL of heparin.

    Endotoxin

    < 1.0 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles

     

    Cautions

     

    1. Avoid repeated freeze-thaw cycles

    2. For your safety and health, please wear lab coats and disposable gloves for operation.

    3. For research use only.

     

    HB220309