- IVD PCR系列
Recombinant Human TNF Ligand-related Molecule 1/TNFSF15 (Human TL-1A/TNFSF15)
TL-1A belongs to the TNF superfamily of ligands. It is expressed predominantly in endothelial cells, and to a lesser extent in the placenta, lung, kidney, skeletal muscle, pancreas, small intestine and colon. TL-1A inhibits endothelial cell proliferation and angiogenesis, and has been shown to induce NF-κB activation, caspase activity, and apoptosis in responding cell lines. TL-1A interacts with TNFRSF25/DR3 receptor, but can also bind to a decoy receptor TNFRSF21/DR6. Recombinant Human TL-1A is a 22.0 kDa polypeptide of 194 amino acid residues.
E.coli-derived Human TL-1A protein,Leu22-Leu251.
Approximately 20.5 kDa
LKGQEFAPSH QQVYAPLRAD GDKPRAHLTV VRQTPTQHFK NQFPALHWEH ELGLAFTKNR MNYTNKFLLI PESGDYFIYS QVTFRGMTSE CSEIRQAGRP NKPDSITVVI TKVTDSYPEP TQLLMGTKSV CEVGSNWFQP IYLGAMFSLQ EGDKLMVNVS DISLVDYTKE DKTFFGAFLL
Sterile Filtered White lyophilized (freeze-dried) powder.
> 97 % by SDS-PAGE and HPLC analyses.
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce apoptosis using human TF-1 cells is less than 20 ng/mL, corresponding to a specific activity of > 5.0 × 104 IU/mg.
< 0.1 EU per 1μg of the protein by the LAL method.
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 0.02% Tween-20.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
1. Avoid repeated freeze-thaw cycles
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.