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400-6111-883
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400-6111-883
Recombinant Human TNF Ligand-related Molecule 1/TNFSF15 (Human TL-1A/TNFSF15)
产品货号:90616ES08
产品规格:5μg
产品价格:¥ 815.00
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Human TNF Ligand-related Molecule 1/TNFSF15 (Human TL-1A/TNFSF15)

    90616ES08

    5μg

    815.00

    90616ES60

    100μg

    7865.00

    90616ES76

    500μg

    20425.00

     

    Product Description

     

    TL-1A belongs to the TNF superfamily of ligands. It is expressed predominantly in endothelial cells, and to a lesser extent in the placenta, lung, kidney, skeletal muscle, pancreas, small intestine and colon. TL-1A inhibits endothelial cell proliferation and angiogenesis, and has been shown to induce NF-κB activation, caspase activity, and apoptosis in responding cell lines. TL-1A interacts with TNFRSF25/DR3 receptor, but can also bind to a decoy receptor TNFRSF21/DR6. Recombinant Human TL-1A is a 22.0 kDa polypeptide of 194 amino acid residues.

     

    Product Properties

     

    Synonyms

    Human TL-1A

    Accession

    O95150

    GeneID

    Source

    E.coli-derived Human TL-1A protein,Leu22-Leu251.

    Molecular Weight

    Approximately 20.5 kDa

    AA Sequence

    LKGQEFAPSH QQVYAPLRAD GDKPRAHLTV VRQTPTQHFK NQFPALHWEH ELGLAFTKNR MNYTNKFLLI PESGDYFIYS QVTFRGMTSE CSEIRQAGRP NKPDSITVVI TKVTDSYPEP TQLLMGTKSV CEVGSNWFQP IYLGAMFSLQ EGDKLMVNVS DISLVDYTKE DKTFFGAFLL

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 97 % by SDS-PAGE and HPLC analyses.

    Biological Activity

    Fully biologically active when compared to standard. The ED50 as determined by its ability to induce apoptosis using human TF-1 cells is less than 20 ng/mL, corresponding to a specific activity of > 5.0 × 104 IU/mg.

    Endotoxin

    0.1 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 0.02% Tween-20.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20 to -80 for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.


    Cautions

     

    1. Avoid repeated freeze-thaw cycles

    2. For your safety and health, please wear lab coats and disposable gloves for operation.

    3. For research use only.

     

    HB220309