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400-6111-883
Recombinant Human Fatty-acid-binding Protein 2 (Human FABP2)
产品货号:92578ES08
产品规格:5 μg
产品价格:¥ 815.00
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Human Fatty-acid-binding Protein 2 (Human FABP2)

    92578ES08

    5 μg

    815.00

    92578ES60

    100 μg

    7865.00

    92578ES76

    500 μg

    20425.00

     

    Product Description

     

    Fatty acid binding protein-2 (FABP-2; also named I- or intestinal FABP) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner. FABP-2 is one of nine cytoplasmic FABPs that are 14-15 kDa in size and range from 126-134 amino acids (aa) in length. Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. Nevertheless, based on aa sequence, the nine FABP family members have been shown to form three subgroups, with FABP2/IFABP linked with liver/L-FABP and heart/H-FABP. The designation of a tissue type, such as intestinal, does not suggest the binding protein is universally expressed in all cell types that make up the organ or tissue. Human I-FABP, the product of the FABP-2 gene, is a 132 aa cytosolic protein that shows a flattened beta -barrel structure (called a beta -clam) generated by a series of antiparallel beta -strands and two alpha helices. Preferred ligands for FABP-2 include sixteen to twenty carbon long chain fatty acids. It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in "internalization" of the ligand. An Ala-to-Thr polymorphism at position #54 has been reported to potentially impact FABP-2 function. This polymorphism has been suggested to be associated with an increased risk of type II diabetes. To date, the evidence appears to be equivocal. This polymorphism may, however, have unusual metabolic effects depending upon the type of diet involved. Human FABP-2 is 78%, 82% and 86% aa identical to mouse, rat and canine FABP-2, respectively. It also shows 33% and 24% aa identity to human H-FABP and LFABP, respectively. FABP-2 is proposed to transport fatty acids (FA) into cells, increase FA availability to enzymes, protect cell structures from FA attack, and target FA to transcription factors in the nuclear lumen.

     

    Product Properties

     

    Synonyms

    IFABP; I-FABP

    Accession

    P12104

    GeneID

    2169

    Source

    E.coli-derived Human FABP2, Ala2-Asp132.

    Molecular Weight

    Approximately 15.1 kDa.

    AA Sequence

    AFDSTWKVDR SENYDKFMEK MGVNIVKRKL AAHDNLKLTI TQEGNKFTVK ESSAFRNIEV VFELGVTFNY NLADGTELRG TWSLEGNKLI GKFKRTDNGN ELNTVREIIG DELVQTYVYE GVEAKRIFKK D

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 97 % by SDS-PAGE and HPLC analyses.

    Biological Activity

    Data Not Available.

    Endotoxin

    0.1 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.0.


    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    Avoid repeated freeze-thaw cycles.

    For your safety and health, please wear lab coats and disposable gloves for operation.

    For research use only!

     

    HB220304