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400-6111-883
Recombinant Human Vimentin (Human Vimentin)
产品货号:92575ES60
产品规格:100 μg
产品价格:¥ 1855.00
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Human Vimentin (Human Vimentin)

    92575ES60

    100 μg

    1855.00

    92575ES76

    500 μg

    7495.00

     

    Product Description

     

    Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells. The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers. Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10-12 nm wide) filaments. These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress. IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis. Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons. Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell. It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge. Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis. Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages. Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst. Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes.

     

    Product Properties

     

    Synonyms

    FLJ36605, VIM

    Accession

    P08670

    GeneID

    7431

    Source

    E.coli-derived Human Vimentin, Ser2-Glu466.

    Molecular Weight

    Approximately 53.5 kDa.

    AA Sequence

    STRSVSSSSY RRMFGGPGTA SRPSSSRSYV TTSTRTYSLG SALRPSTSRS LYASSPGGVY ATRSSAVRLR SSVPGVRLLQ DSVDFSLADA INTEFKNTRT NEKVELQELN DRFANYIDKV RFLEQQNKIL LAELEQLKGQ GKSRLGDLYE EEMRELRRQV DQLTNDKARV EVERDNLAED IMRLREKLQE EMLQREEAEN TLQSFRQDVD NASLARLDLE RKVESLQEEI AFLKKLHEEE IQELQAQIQE QHVQIDVDVS KPDLTAALRD VRQQYESVAA KNLQEAEEWY KSKFADLSEA ANRNNDALRQ AKQESTEYRR QVQSLTCEVD ALKGTNESLE RQMREMEENF AVEAANYQDT IGRLQDEIQN MKEEMARHLR EYQDLLNVKM ALDIEIATYR KLLEGEESRI SLPLPNFSSL NLRETNLDSL PLVDTHSKRT LLIKTVETRD GQVINETSQH HDDLE

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 95% by SDS-PAGE and HPLC analyses.


    Biological Activity

    Data Not Available.

    Endotoxin

    0.1 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 µm filtered solution in 30 % Acetonitrile and 0.1 % TFA.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    Avoid repeated freeze-thaw cycles.

    For your safety and health, please wear lab coats and disposable gloves for operation.

    For research use only!

     

    HB220302