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400-6111-883
Recombinant Human Desert Hedgehog Cys23IleIle (Human DHH C23II)
产品货号:92570ES08
产品规格:5 μg
产品价格:¥ 815.00
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Human Desert Hedgehog Cys23IleIle (Human DHH C23II)

    92570ES08

    5 μg

    815.00

    92570ES60

    100 μg

    7185.00

    92570ES76

    500 μg

    18935.00

     

    Product Description

     

    Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Desert Hedgehog is a secreted, 45 kDa, 373 amino acid (aa) protein that undergoes autocatalytic cleavage between Gly198 and Cys199 catalyzed by the C-terminal domain, which releases the N-terminal domain with a concominant attachment of cholesterol at its new C-terminus. In addition to the C-terminally attached cholesterol, a fatty acid acyl chain is esterified to the N-terminal cysteine (aa 23) via an amide linkage. The 19 kDa N-terminal signaling domain is membrane associated due to its double lipid modifications. Its binding to Patched receptors results in the loss of Patched repression of Smoothened signaling. Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip). Within the N-terminal domain, human Dhh shares 97% aa sequence identity with mouse and rat Dhh. It shares approximately 75% aa seqeuence identity with human Indian (Ihh) and Sonic Hedgehog (Shh). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis. It induces steroidogenic factor 1, which is instrumental in promoting Leydig cell differentiation. It also promotes the deposition of basal lamina surrounding seminiferous tubules. Mutations of Dhh are linked to complete pure gonadal dysgenesis. Dhh is expressed in the female by ovarian granulosa cells and the corporus luteum. Its upregulation in ovarian cancer correlates positively with proliferative index, and negatively with prognosis. Dhh is also expressed by Schwann cells and is upregulated following nerve injury. It induces the expression of Patched and Hip in neural tissue fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves.

     

    Product Properties

     

    Synonyms

    DHH; HHG-3MGC35145

    Accession

    O43323

    GeneID

    50846

    Source

    E.coli-derived Human DHH C23II, Cys23-Gly198.

    Molecular Weight

    Approximately 19.9 kDa.

    AA Sequence

    IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 96% by SDS-PAGE and HPLC analyses.

    Biological Activity

    The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2 (CCL-226) cells is 15-45 μg/mL. Fully biologically active when compared to standard.

    Endotoxin

    < 1.0 EU per 1μg of the protein by the LAL method.


    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in 10mM PB, pH 6.0, 300mM NaCl.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    Avoid repeated freeze-thaw cycles.

    For your safety and health, please wear lab coats and disposable gloves for operation.

    For research use only!

     

    HB220304