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400-6111-883
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400-6111-883
Recombinant Rat Migration Inhibitor Factor (Rat MIF)
产品货号:92510ES10
产品规格:10 μg
产品价格:¥ 815.00
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥)

     

    Recombinant Rat Migration Inhibitor Factor (Rat MIF)

     

    92510ES10

    10 μg

    815.00

    92510ES60

    100 μg

    7865.00

    92510ES76

    500 μg

    20425.00

     

    Product Description

     

    Macrophage migration inhibitory factor (MIF or MMIF), also named as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase, is a protein encoded by the MIF gene. It is released from white blood cells by bacterial antigen stimulation to trigger an acute immune response, or by glucocorticoids to counter-act the inhibitory effects of glucocorticoids on immune system. MIF is a homotrimer of which each subunit contains 115 amino acids. As mentioned above, MIF is involved in the innate immune response to bacterial pathogens and counter-acts the anti-inflammatory activity of glucocorticoids. Furthermore, it also plays a role as mediator in regulating the function of macrophages in host defense and has phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro. Rat MIF is 99 %, 90 %, 89 %, and 89 % a.a. identical to human, murine, porcine and bovine, respectively.

     

    Product Properties

     

    Synonyms

    MIF, Glutathione-binding 13 kDa Protein, L-dopachrome Isomerase, L-dopachrome Tautomerase, Phenylpyruvate Tautomerase

    Accession

    GeneID

    Source

    E.coli-derived Rat MIF, with an N-terminal Met.

    Molecular Weight

    Approximately 12.5 kDa

    AA Sequence

    MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 97 % by SDS-PAGE and HPLC analyses.

    Biological Activity

    Test in process.

    Endotoxin

    < 1.0 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, pH 8.0, 150 mM NaCl, 3 % trehalose.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions


    Avoid repeated freeze-thaw cycles.

    For your safety and health, please wear lab coats and disposable gloves for operation.

    For research use only!


    HB220301