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400-6111-883
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400-6111-883
Recombinant Human Neurotrophin-4 (Human NT-4)
产品货号:92114ES10
产品规格:10 μg
产品价格:¥ 1855.00
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Human Neurotrophin-4 (Human NT-4)

    92114ES10

    10 μg

    1855.00

    92114ES60

    100 μg

    13615.00

    92114ES76

    500 μg

    35395.00

     

    Product Description

     

    Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. The human NT-4 cDNA encodes a 210 amino acid (aa) precursor that includes a 24 aa signal sequence, a 56 aa propeptide, and a 130 aa mature protein. NT-4 is synthesized as a 28 kDa prepropeptide that is proteolytically processed to generate the mature protein. Mature human NT-4 shares 48-52% aa sequence identity with human beta-NGF, BDNF, and NT-3. It shares 91% and 95% aa sequence identity with mouse and rat NT-4/5, respectively. The mature protein is secreted as a homodimer and can also form heterodimers with BDNF or NT-3. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 promotes the development and survival of selected peripheral and CNS neurons. BDNF, which also activates TrkB, overlaps with many but not all NT-4 functions, a distinction that is likely due to differences in expression patterns. NT-4 induced TrkB signaling augments NMDA receptor activity and increases neuronal sensitivity to excitotoxic cell death. It also promotes the proliferation of keratinocytes and accelerates hair follicle regression during the follicular cycle. NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration.

     

    Product Properties

     

    Synonyms

    Neurotrophin-5, NT-5

    Accession

    P34130

    GeneID

    4909

    Source

    E.coli-derived Human NT-4, G81-A210, with an N-terminal Met.

    Molecular Weight

    Approximately 28.1 kDa.

    AA Sequence

    MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 97 % by SDS-PAGE and HPLC analyses.

    Biological Activity

    The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50 ng/mL, corresponding to a specific activity of > 2.0 × 104 IU/mg. Fully biologically active when compared to standard. 

    Endotoxin

    1.0 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.5.


    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    Avoid repeated freeze-thaw cycles.

    For your safety and health, please wear lab coats and disposable gloves for operation.

    For research use only!

     

    HB220309