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400-6111-883
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400-6111-883
Recombinant Human Fibroblast Growth Factor 12 (Human FGF-12)
产品货号:91307ES10
产品规格:10 μg
产品价格:¥ 1855.00
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Human Fibroblast Growth Factor 12 (Human FGF-12)

    91307ES10

    10 μg

    1855.00

    91307ES60

    100 μg

    7715.00

    91307ES76

    500 μg

    20315.00

     

    Product Description

     

    Fibroblast growth factor 12 (FGF12) is a fibroblast growth factor homologous factor, a subset of the FGF superfamily. Human FGF-12 is synthesized as a 243 aa protein. In human Vascular smooth muscle cells (VSMCs), FGF12 expression was inhibited at the transcriptional level by platelet-derived growth factor-BB. FGF12 inhibited cell proliferation through the p53 pathway and upregulated the key factors involved in VSMC lineage differentiation, such as myocardin and serum response factor. In addition, FGF12 and other FGF11 subfamily members do not activate any fibroblast growth factor receptors (FGFRs), although they can bind to heparin with high affinity like other FGFs. FGF12 has structural similarity with FGF1 and FGF2, in that it lacks a classical signal sequence and con-tains a nuclear localization signal, resulting in the accu-mulation of FGF12 in the nucleus without any release from cells.

     

    Product Properties

     

    Synonyms

    FGF12, FGF-12, FGF12B, FHF1, FHF-1, fibroblast growth factor 12, fibroblast growth factor 12B

    Accession

    GeneID

    2257

    Source

    E.coli-derived human FGF-12 protein, Met1-Thr181.

    Molecular Weight

    Approximately 20.5 kDa.

    AA Sequence

    MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK ASLYVAMNGE GYLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ IMKGNRVKKT KPSSHFVPKP IEVCMYREQS LHEIGEKQGR SRKSSGTPTM NGGKVVNQDS T

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    >98% by SDS-PAGE and HPLC analyses.

    Biological Activity

    The biological activity was determined by its binding ability in a functional ELISA. Immobilized recombinant human FGF R4/Fc Chimera at 5 µg/mL (100 µL/well) can bind recombinant human FGF-12 with a linear range of 1.6-100 ng/mL.

    Endotoxin

    0.1 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4, with 1 mM DTT.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.


    Cautions

     

    Avoid repeated freeze-thaw cycles.

    For your safety and health, please wear lab coats and disposable gloves for operation.

    For research use only!

     

    HB220303