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400-6111-883
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400-6111-883
Recombinant Human Interleukin-8, 72aa/CXCL8 (Human IL-8,72aa/CXCL8)
产品货号:90189ES08
产品规格:5 μg
产品价格:¥ 815.00
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥)

    Recombinant Human Interleukin-8, 72aa/CXCL8 (Human IL-8,72aa/CXCL8)

    90189ES08

    5 μg

    815.00

    90189ES60

    100 μg

    7865.00

    90189ES76

    500 μg

    20425.00

     

    Product Description

     

    Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1, is a widely expressed proinflammatory member of the CXC family of chemokines. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). IL-8/CXCL8 can associate into a homodimer or a heterodimer with CXCL4/PF4 (2), and it can interact with matrix and cell surface glycosaminoglycans. Near its N-terminus, this 8-9 kDa chemokine contains an ELR motif which is important for its angiogenic properties. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. In addition, IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer. It induces VEGF expression, vascular endothelial cell proliferation, angiogenesis, and tumor cell invasiveness.

     

    Product Properties

     

    Synonyms

    (Ser-IL-8)72, GCP/IL-8 protein I, IL8/NAP1 form III, LYNAP, MDNCF-c, NAF

    Accession

    P10145

    GeneID

    3576

    Source

    E.coli-derived Human IL-8,72aa/CXCL8, Ser28-Ser99.

    Molecular Weight

    Approximately 8.4 kDa.

    AA Sequence

    SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 97% by SDS-PAGE and HPLC analyses.



    Biological Activity

     The ED50 as determined by a chemotaxis bioassay using human CXCR2 transfected mouse BaF3 cells is less than 2 ng/mL, corresponding to a specific activity of > 5.0 × 105 IU/mg. Fully biologically active when compared to standard.

    Endotoxin

    < 1.0 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    Avoid repeated freeze-thaw cycles.

    For your safety and health, please wear lab coats and disposable gloves for operation.

    For research use only!

     

    HB220225