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400-6111-883
Recombinant Human Interleukin-36 gamma, 169aa (Human IL-36γ,169aa)
产品货号:90128ES10
产品规格:10 μg
产品价格:¥ 1855.00
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Human Interleukin-36 gamma, 169aa (Human IL-36γ,169aa)

    90128ES10

    10 μg

    1855.00

    90128ES60

    100 μg

    13615.00

    90128ES76

    500 μg

    35395.00

     

    Product Description

     

    IL-36 gamma [previously called IL-1F9, IL-1 epsilon (epsilon), and IL-1H1] is a member of the IL-1 family which includes IL1 beta, IL1 alpha, IL1ra, IL18, IL36 Ra (IL1F5), IL-36 alpha (IL1F6), IL36 beta (IL1F8), IL37 (IL1F7) and IL-1F10. All family members show a 12 beta strand, beta trefoil configuration, and are believed to have arisen from a common ancestral gene. IL36 gamma is an 1822 kDa, 169 amino acid intracellular and secreted protein that contains no signal sequence, no prosegment and no potential Nlinked glycosylation sites. Human IL36 gamma (aa 18169) shares 58%, 59%, 68% and 69% aa sequence identity with mouse, rat, bovine and equine IL36 gamma, respectively, and 2357% aa sequence identity with other family members. 134 aa isoform missing aa 1953 has been reported. Highest levels of IL36 gamma are produced by Langerhans cells, keratinocytes, and stomach Chief cells and parietal cells; these cells contribute to firstline defense against pathogens in the skin, lungs and digestive tract. Its expression is induced by LPS treatment of monocytes, and by IL alpha / beta, IL17 or TNF alpha treatment of keratinocytes and bronchial epithelia. Skin IL-36 gamma expression is increased in contact hypersensitivity and psoriasis. It is elevated in inflammatory disorders of the lung (such as asthma) and viral infections. Lung IL36 gamma and other IL36 proteins contribute to neutrophil influx. The receptor for IL-36 gamma is a combination of IL1 Rrp2, mainly found in epithelia and keratinocytes, and the widely expressed IL1 RAcP. IL36 alpha,beta and gamma all activate NF kappa B and MAPK pathways in an IL1 Rrp2 dependent manner, and IL36 gamma induces production of inflammatory cytokines and chemokines such as CXCL8/IL8.

     

    Product Properties

     

    Synonyms

    IL-1RP2, IL-1 epsilon, IL-1F9, IL-1H1

    Accession

    GeneID

    Source

    E.coli-derived human Interleukin-36 gamma protein,Met1-Asp169,with an N-terminal Met.

    Molecular Weight

    Approximately 18.7 kDa.

    AA Sequence

    MRGTPGDADG GGRAVYQSMC KPITGTINDL NQQVWTLQGQ NLVAVPRSDS VTPVTVAVIT CKYPEALEQG RGDPIYLGIQ NPEMCLYCEK VGEQPTLQLK EQKIMDLYGQ PEPVKPFLFY RAKTGRTSTL ESVAFPDWFI ASSKRDQPII LTSELGKSYN TAFELNIND

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 95% by SDS-PAGE and HPLC analyses.

    Biological Activity

    Fully biologically active when compared to standard. The specific activity is determined by its binding ability in a functional ELISA. Immobilized rHuIL-36γ at 1 µg/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 µg/mL.

    Endotoxin

    < 1.0 EU per 1μg of the protein by the LAL method.


    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    Avoid repeated freeze-thaw cycles.

    For your safety and health, please wear lab coats and disposable gloves for operation.

    For research use only.

     

    HB220225