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400-6111-883
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400-6111-883
Recombinant Rat Stem Cell Factor (Rat SCF)
产品货号:92268ES10
产品规格:10 μg
产品价格:¥ 1855
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Rat Stem Cell Factor (Rat SCF)

    92268ES10

    10 μg

    1855.00

    92268ES60

    100 μg

    7865.00

    92268ES76

    500 μg

    20425.00

     

    Product Description

     

    Stem cell factor (SCF) is a potent hematopoietic growth factor required in regulating both embryonic and adult hematopoiesis. Mature stem cell factor consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane domain, and a 36 aa cytoplasmic tail. Human SCF protein shares 79% 87% aa sequence identity with dog, cat, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. SCF is a primary growth and activation factor for mast cells and eosinophils. Stem cell factor was demonstrated to be the ligand for the c-kit tyrosine kinase receptor. In the mouse, SCF is encoded by Sl (steel), a gene critical to the development of several distinct cell lineages during embryonic life and which has important effects on hematopoiesis in the adult animal.

     

    Product Properties

     

    Synonyms

    FPH2, FPHH, Hematopoietic growth factor KL, MGF, SHEP7, SLF

    Accession

    GeneID

    Source

    E.coli-derived rat SCF protein, Gln26-Ala189, with an N-terminal Met.

    Molecular Weight

    Approximately 18.5 kDa.

    AA Sequence

    MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 95% by SDS-PAGE and HPLC analyses.

    Biological Activity

    The ED50 as determined by a cell proliferation assay using murine MC/9-2 mast cells is less than 5 ng/mL, corresponding to a specific activity of > 2.0 × 105 IU/mg. Fully biologically active when compared to standard.

    Endotoxin

    < 1.0 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.


    Cautions

     

    Avoid repeated freeze-thaw cycles.

    For your safety and health, please wear lab coats and disposable gloves for operation.

    For research use only!

     

    HB220214