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400-6111-883
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400-6111-883
Recombinant Human Transforming Growth Factor - beta 1 (Human TGF-β1)
产品货号:91701ES08
产品规格:5μg
产品价格:¥ 885
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Human Transforming Growth Factor - beta 1 (Human TGF-β1)

    91701ES08

    5μg

    885.00

    91701ES60

    100μg

    13615.00

     

    Product Description

     

    Transforming growth factor-beta1 (TGF-β1) is a major pluripotential cytokine with a pronounced immunosuppressive effect. TGF-β1 is one of three closely related mammalian members of the large TGF-β superfamily that share a characteristic cystine knot structure. Mature human TGF-β1 shares 100% aa identity with pig, dog and cow TGF-β1, and 99% aa identity with mouse, rat and horse TGF-β1. TGF-β1 is a key regulator of diverse biological processes in many tissues and cell types, its can be tumorsuppressive through the activation of the Smadmediated signaling pathway. TGF-β1 inhibits the growth factor and oncogene induction of transin/stromelysin, a secreted matrix-degrading metalloprotease. 

     

    Product Properties

     

    Synonyms

    CEDLAP, DPD1, latency-associated peptide, TGF beta, TGF beta1, TGFB, TGFB1

    Accession

    GeneID

    7040

    Source

    CHO-derived Human TGF-β1, Ala279-Ser390.

    Molecular Weight

    Approximately 12 kDa under reducing conditions.

    AA Sequence

    ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    >97% by SDS-PAGE analyses.

    Biological Activity

    Measured by its ability to inhibit the IL-4-dependent proliferation of HT2 mouse T cells. The ED50 for this effect is 0.04-0.2 ng/mL. The specific activity of Recombinant Human TGF-β1 is approximately 2.5 × 104 U/μg, which is calibrated against human TGF-β1 Standard.

    Endotoxin

    0.1 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in 35% Acetonitrile and 0.1% TFA.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriately buffered solutions.

     

    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions


    Avoid repeated freeze-thaw cycles.

    For your safety and health, please wear lab coats and disposable gloves for operation.

    For research use only!

     

    HB220215