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400-6111-883
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400-6111-883
Recombinant Human Fibroblast growth factor 19 (Human FGF-19)
产品货号:91311ES08
产品规格:5 μg
产品价格:¥ 815
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Human Fibroblast growth factor 19 (Human FGF-19)

    91311ES08

    5 μg

    815.00

    91311ES60

    100 μg

    7865.00

    91311ES76

    500 μg

    20425.00

     

    Product Description

     

    Fibroblast growth factor 19 (FGF-19) belongs to the large FGF family which has at least 23 members. Human FGF-19 is most closely related to chicken FGF-19 and murine FGF-15, sharing approximately 61% and 51% aa sequence identity, respectively. The FGF19-FGFR4 signaling axis has been implicated in the pathogenesis of several cancers, including hepatocellular carcinomas in mice and potentially in humans. FGF-19 differs from the classical FGFs in that it has a much-reduced heparan sulfate proteoglycan binding affinity that allows it to act as endocrine hormone. Although FGF-19 regulates several different metabolic activities, it still activates downstream signaling pathways through FGF receptors, in a similar manner to that seen in classical FGFs. 

     

    Product Properties

     

    Synonyms

    FGF19, FGF-19, fibroblast growth factor 19 

    Accession

    GeneID

    Source

    E.coli-derived human FGF-19, Arg23-Lys216, with an N-terminal Met.

    Molecular Weight

    Approximately 21.8 kDa.

    AA Sequence

    MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    >95% by SDS-PAGE and HPLC analyses.

    Biological Activity

    1: The biological activity is measured by its binding ability in a functional ELISA. Immobilized recombinant human FGF R4 at 5 µg/mL can bind recombinant human FGF-19 with a linear range of 3-200 ng/mL. Fully biologically active when compared to standard.
    2: The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 150 ng/mL, corresponding to a specific activity of > 6.7 × 103 IU/mg. Fully biologically active when compared to standard.

    Endotoxin

    1.0 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.


    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    Avoid repeated freeze-thaw cycles.

    For your safety and health, please wear lab coats and disposable gloves for operation.

    For research use only!

     

    HB220215