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400-6111-883
Recombinant Human Fms-related Tyrosine Kinase 3 Ligand(Human Flt-3 Ligand)
产品货号:92259ES10
产品规格:10 μg
产品价格:¥ 1855
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Human Fms-related Tyrosine Kinase 3 Ligand(Human Flt-3 Ligand)

    92259ES10

    10 μg

    1855.00

    92259ES60

    100 μg

    10215.00

    92259ES76

    500 μg

    26545.00


    Product Description

     

    The cytokine Fms-like tyrosine kinase 3 ligand (FLT3L), also known as Flt-3 Ligand, is an important regulator of hematopoiesis. Its receptor, Flt3, is expressed on myeloid, lymphoid and dendritic cell progenitors and is considered an important growth and differentiation factor for several hematopoietic lineages. Activating mutations of Flt3 are frequently found in acute myeloid leukemia (AML) patients and associated with a poor clinical prognosis. Mature human Flt3L consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. At the amino acid sequence level, human and mouse FL are approximately 72% identical and the two proteins exhibit cross-species activity. The Flt3-L manifests antitumor activity, presumably due to its capacity to recruit dendritic cells and cause their proliferation.

     

    Product Properties

     

    Synonyms

    Flt3-Ligand, FL, FLG3L, Flt3L, SL Cytokine

    Accession

    GeneID

    Source

    E.coli-derived human IL-1alpha protein, Thr27-Ala181.

    Molecular Weight

    Approximately 17.6 kDa.

    AA Sequence

    TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 97% by SDS-PAGE and HPLC analyses.

    Biological Activity

    The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 × 106 IU/mg. Fully biologically active when compared to standard.

    Endotoxin

    < 1.0 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.


    Shipping and Storage

     

    The products are shipped with ice pack and can be stored at -20 to -80 for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    Avoid repeated freeze-thaw cycles.

    For your safety and health, please wear lab coats and disposable gloves for operation.

    For research use only!

     

    HB220210