- IVD PCR系列
Recombinant Human LR3 Insulin-like Growth factor-1,Media Grade (Human LR3 IGF-1)
Long R3 IGF-I (LR3 IGF-I) is a 9.2 kDa synthetic analog of IGF-I that is generated by modifying the aa sequence for mature human IGF-I. The LR3IGF-1 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals. LR3 IGF-I, an analogue of insulin-like growth factor (IGF)-I, was used in biopharmaceutical protein production in mammalian cells. And the body growth of animals is regulated by growth hormone and IGF-I. The classical theory of this regulation is that most IGF-I in the blood originates in the liver and that body growth is controlled by the concentration of IGF-I in the blood. Meanwhile, IGF-I has been shown to enhance neuronal survival and inhibit apoptosis.
LR3 IGF-I, IBP1, IGF1, IGF-1, IGF1A, IGFI, IGF-I, IGF-IA, IGF-IB, MGF
E.coli-derived human LR3 IGF-1 protein, Gly49-Ala118.
Approximately 9.1 kDa.
MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA
Sterile Filtered White lyophilized (freeze-dried) powder.
>98% by SDS-PAGE analyses.
>90% by RP-HPLC analyses.
1: Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED50 for this effect is 0.3-1.5 ng/mL, corresponding to a specific activity of > 6.7 × 105 U/mg.
2:The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/mL, corresponding to a specific activity of > 1.0 × 105 U/mg. Fully biologically active when compared to standard.
< 0.01 EU per 1μg of the protein by the LAL method.
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.
For research use only.