- IVD PCR系列
Recombinant Human Interferon-gamma(Human IFN-γ)
Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a pleiotropic cytokine which is now recognised as an important modulator of the immune response. Mature human IFN-γ exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits. It shares 90% amino acid (aa) sequence identity with rhesus IFN-γ, 59%64% with bovine, canine, equine, feline, and porcine IFN-γ, and 37%43% with cotton rat, mouse, and rat IFN-γ. Interferons have had increasing clinical usage in the treatment of a variety of disorders, at times being used in combination with chemo‐ or radiotherapy.IFN-γ increases the potential immunogenicity of vascular endothelial cells by up-regulation of intercellular adhesion molecule-1 (ICAM-1) and class I MHC antigen expression and by induction of class II MHC antigens and certain chemokines.
Interferon-gamma, IFN-γ, IFG, IFI, IFNG, IFNgamma, IFN-gamma.
E.coli-derived human IFN-γ protein, Gln24-Gln166 with an N-terminal Met.
Approximately 16.9 kDa.
MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ
Sterile Filtered White lyophilized (freeze-dried) powder.
> 98 % by SDS-PAGE and HPLC analyses.
The ED50 as measured in anti-viral assays using human HeLa cells infected with encephalomyocarditis (EMC) virus is 0.15-0.80 ng/ml. Fully biologically active when compared to standard.
< 1.0 EU per 1μg of the protein by the LAL method.
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.
For research use only!